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KMID : 1188320090030020088
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Gut and Liver
2009 Volume.3 No. 2 p.88 ~ p.94
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The Clinicopathological Significance of Tissue Levels of Hypoxia-Inducible Factor-1? and Vascular Endothelial Growth Factor in Gastric Cancer
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Kim Seong-Eun
Shim Ki-Nam
Jung Sung-Ae
Yoo Kwon
Lee Joo-Ho
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Abstract
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Background/Aims: Hypoxia-inducible factor-1? (HIF- 1?) is a mediator of tumor progression. Vascular endothelial growth factor (VEGF) is the most potent angiogenic factor known to be induced by HIF-1?. We investigated the clinicopathological significance of HIF-1? and VEGF levels in biopsied gastric cancer tissue.
Methods: Endoscopic biopsy specimens from 67 patients with gastric carcinoma who underwent surgery were available for this study. Semiquantitative RT-PCR was applied to biopsied tumors and normal tissues to determine the expressions of HIF-1? and VEGF. The expression levels of HIF-1? and VEGF were evaluated using the tumor:normal (T/N) ratios of HIF-1? and VEGF mRNA. The clinicopathological variables were reviewed retrospectively.
Results: The T/N ratios of HIF-1? mRNA showed significant correlation with lymph-node metastases, distant metastases, stage, and recurrence within 3 years (p＜0.05). The T/N ratios of VEGF mRNA showed significant correlation with lymph-node metastases and distant metastases (p＜0.05). There was a significant correlation between the T/N ratios of HIF-1? and VEGF mRNA (r=0.72, p＜0.01).
Conclusions: The increased expression of HIF-1? and VEGF mRNA could reflect aggressive tumor behavior, including the recurrence of gastric cancer. Examination of HIF-1? mRNA in biopsy specimens by RT-PCR assay might provide useful preoperative information on tumor aggressiveness.
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KEYWORD
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Stomach neoplasms, Hypoxia-inducible factor 1, alpha, Vascular endothelial growth factor
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